PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency
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چکیده
منابع مشابه
PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency
This study addresses the problem of PCR inhibition by reverse transcriptase. It has been shown that the inhibition occurs mostly when a small amount of RNA is taken for RT reaction, and it is more visible for rarely expressed transcripts. We show here that the inhibition takes place regardless of what amount of template is utilized for RT. The inhibition possesses a global nature, i.e. the ampl...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2005
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gni176